Hawaii Tolerance of PI Curve

##Daily accomplishment

18 september 2023:

Unpacking suplies and starting to setup the PI Curve TPC station. Thinking about the plan and what do we need to do day per day to finish the filed trip in time.

###19 september 2023:

  • Field: We want in the field at the reef 30 (HIMB localisation) for collecting corals to run all the PI Curve TPC. We went in the filed between 8:30 and 9:45am and collected colonies of Montipora capitata, Porites compressa and Pocillopora acuta. We collected 8 genotype for each species around the same area. Unfortunetly with confusion we miss one colonie of P.acuta.

  • Wet Lab / Coral table:
    We taged 10 plug for each colonie with the letter A,B,C,D,E,F,G and H. Futher we fragment and glue each colonies on the plug create for (following the Genotype letter and number (1 to 10)) between 5:00 and 5:30pm.

20 september 2023

Wet Lab / Daily mesurment: Load the Hobo loger : Incubator 2 start at 12:10 - Control tank 2 start at 12:10 Put in the water at 12:09

####15:45#### Daily mesurment and add salinity, pH. The light mesurment was disturb because of the sky (cloudy).

####16:30 to 17:45#### Start to plug extras fragments for different colony. Not all teh colony have fragment in addition.
P. compressa add F11, F12, F13,F14 / G11, G12, G13, G14 and E11, E12, E13, E14, E15, E16.

M. capitata add A11, A12, A13, A14, A15, A16, A17, A18, A19, A20, A21 / D11, D12, D13 and F11, F12, F13, F14, F15, F16.

P. acuta add G11, G12, G13, G14 / C11, C12, C13, C14, C15, C16, C17, C18, C19, C20 / B11, B12, B13, B14 and A11, A12, A13, A14.

####Set up PICURVE TPC system Disposition of the stirplates

1 2 3 X 1 2 3
4 5 6 X 4 5 6
7 8 9 X 7 8 9
10 11 12 X 10 11 12
13 14 15 X 13 14 15
TPC1 TPC1 TPC1 X TPC2 TPC2 TPC2

Set 12 AI Prime 16HD Reef lights above TPC tanks.
Tested light levels with PARWISE %settings all the same expect UV=0%

  100% 100% 10% 10% 5% 5%
  TPC1 TPC2 TPC1 TPC2 TPC1 TPC2
1 839 1192 81 111 31 38
2 915 1114 88 109 33 34
3 1115 954 105 101 38 28
4 904 1320 88 121 34 41
5 994 1218 94 115 35 39
6 1191 1014 112 110 40 37
7 1099 1316 102 126 37 44
8 1108 1179 106 113 40 39
9 1279 988 120 104 44 40
10 1355 1008 128 95 48 33
11 1227 1229 117 121 44 40
12 1355 1008 128 95 48 33
13 1049 1321 121 122 45 44
14 1237 1227 120 113 40 40
15 1324 1035 126 98 46 36

21 september 2023

Written on September 23, 2023