Sample Preparation Protocoles

POC ID SEM lab preparation

Protocol

Haifa

20240409

POC Flow

• Creation of POC Flow experiment project file/folder in drive call “Flow Jordan Experiment”.
• Select pictures and rename each of them by their identification number. All pictures are available on OSF file called “Flow Jordan Experiment”. Structure of the name identification (FLOW _ PT _ 17).

Sample ID problem and need to be fix.

- Sample called ***FLOW _ PG _16*** need to be rename as ***FLOW _ PT _ 16*** on the whirlpak and paper inside the whirlpak.  

##Daily accomplishment

18 september 2023:

Unpacking suplies and starting to setup the PI Curve TPC station. Thinking about the plan and what do we need to do day per day to finish the filed trip in time.

BSF Project

Project and grants between Israel and Hawaii

Overview

As part of the project, we want to experiment with new tiles for coral recruitment, using new technologies such as 3D printers. All tiles are designed and printed by the Israel Institute of Technology. Composed of various components such as CaCO3 (DEFINE), PA12 (DEFINE). Their size will be 1.5cmX1.5cmX0.3cm.

SEM Haplotype characterization | POC_ID

Summary

As part of my PhD project on Settlement and post settlement challenges to successful recruitment for reef building corals the second chapter focused on the relative abundance of Pocillopora sp. within the Moorea lagoon. A characterization study of the calcareous skeleton by Haplotype (Johnston et al 2021) will be carried out. In addition to genetic identification, this study will enable us to understand the plasticity of each Haplotype as a function of its natural environment. It will also highlight the great interspecific diversity of Pocillopora sp. populations. Analysis of the fragments will be carried out at Haifa University using the SEM 3D Imagine technique (Goodbody-Gringley & Waletich, 2018; Doherty, Matthew et al. 2023)

Coral health status protocol.

The health of coral reefs is of paramount importance to millions of people around the world (CITE). Coral reefs provide many ecosystem services, including coastal protection, necessary nutrition, habitat for many animal species, and important economic benefits such as tourism (CITE).

Due to coral bleaching (Hughes et al. 2018), anthropogenic pollution (Bellwood et al. 2004), and ocean acidification (CITE), corals can show signs of poor health and thus lose their natural functions.

Through a health status chart (CITE) set up by the Australia Institute of Marine Biology, we are able to create health status tracking through a color chart.

The following protocol allows the fragmentation of corals to create microfragments, adapting the size of the fragments desired for experimentation and/or coral restoration. Gryphon can be used on both live and dead corals as needed.

ID Pocillopora sp. Haplotype versus color

#Map mapping with Allen Coral Atlas, Mapshaper and R ##Allen Coral Atlas

#Coral spawning observation

Context

Dans la littérature, peut sont les papiers consacré à la reproduction des Pocillopora verrucosa, P.meandrina et P.grandis du à de nombreuses raison tels-que la taille des oeufs générant des problèmes et des diffcultés durant leurs analyses, sensibilité de leurs resistance en aquariums mais également dû à de nombreuses erreur d’identification. Les papiers sont principalement basé sur la grande barriere de corail Australienne (REF).

FieldWork Tetiaroa May 2022. Coral detection for future studies. Pierrick Harnay - Hollie Putnam

Pocillopora sp. morphologie detection

Making NoteBook post on Github step