Pocillopora Transect

Pocillopora sp. morphologie detection

Context of studies

Our study is based on population genetics of Pocillopora sp. on the Polynesian islands based on their often misleading and incorrect morphology. We are therefore trying to understand population dynamics by Haplotype so that we can correlate our transect data with our term tolerance data from the laboratory (see NoteBook TPC Haplotype).

Protocol

Stuff

  • Decametre
  • Gopro
  • Regular cameras
  • Whirl pack with labels
  • Hammer
  • Chisel
  • Measuring rod with color measumnent
  • Mesh Bag for bring sampling corals
  • Cooler (for transport)
  • GPS
  • Cutting pliers (for the recruit)
  • Cooler
  • Quadrats
  • Knief

Field protocol

In the lab

  1. Prepare the necessary material for the old field including
    - Charging the batteries of the camera(s)
    - Charging the batteries of the Gopro(s)
    - Labeled bags for sampling
    - Labeling bags to store samples per transect
    - Double label the sample storage bags by transect with a sticker
    - Verify that all materials are ready to leave the next day (a second verification will be done before leaving for the field)

In the field

  1. Select the sampling locations
  2. Check equipment and compare to above list
  3. Load the boat
  4. Once you arrive on site, define who does what and do not change during the trip to avoid missing any step
  5. Draw the 50 m transect in the desired direction (North, South, West, East)

Transect_example

  1. Using the GPS, take the starting point of the transect as well as the end point
  2. Follow the transect by filming the transect with a Gopro (to keep track of the transect in case of doubt during analysis and/or need for more information on the transect)
  3. Use the photo quadrat and start tracking by quadrats

  4. Placed the quadrats every two meters by having the quadrats centered on the transect in order to have 25cm on each side n=25 (see figure bellow)
    QUADRAT_PROTOCOL

  5. Return to the start of the transect and begin the coral sampling steps
  6. At the beginning of each transect, take a photo of the storage bag so that the photos do not get mixed up during processing.
  7. Take a picture of the colony in the global view and of the colony in the closer view by placing the colored ruler close to the colony (see pictures bellow)
    POC_image

POC-178

POC_TPC

  1. Take a picture of the bag that will contain the sample
  2. Sample a fragment of a branch of about 4-5cm maximum (depending on the size of the colony). Each sample collecting need to be on the transect line or at 25cm to the left or right to the line maximum (see figure bellow)

TRANSECT_PROTOCOL

  1. Close the sample bag
  2. Place the bag in the meshbag assigned to the transect number
  3. Proceed to the next colony
  4. At the end of each transect, take a random photo to separate the transect photos
  5. Proceed to the next transect by repeating the steps listed on the protocol.

When you come back to the field

  1. Put the samples in a freezer at -80°C in case the extraction or other analyses are not carried out directly afterwards.
  2. Rinse used and unused equipment in the field to avoid breakage or wear due to seawater
  3. Repeat the procedure (In the lab) for the next day of sampling
  4. Download the photos taken by transect and file them on transect files, not forgetting all the photos. Each sample must have 3 photos.
  5. Download the GPS data and record the GPS points of the transects in a file

Important point:

1. All colonies of Pocillopora sp. regardless of age or morphology should be collected
2. Each transect must have its own sampling bag to avoid mixing the samples

What has already been done

Map of the studies zone:
All sites on the map below are the sites that will be conducted for the study of population genetics of Pocillopora sp.

MOOREA_RELATIVE_ABUNDANCE

Site GPS position:
All the point bellow matches to red point on the above map

site.name Long Lat
site1 W 149.876483662432 S 17.4852396168452
site2 W 149.852645222058 S 17.4822393349348
site3 W 149.834436650297 S 17.4781017702069
site4 W 149.807646130636 S 17.4756927846393
site5 W 149.783569102487 S 17.4730460839688
site6 W 149.761472375314 S 17.5057595733415
site7 W 149.763693925498 S 17.5180522524175
site8 W 149.764229432436 S 17.5373725115999
site9 W 149.784554761853 S 17.5653531181373
site10 W 149.787804991036 S 17.5792038175915
site11 W 149.804473029377 S 17.6027675188675
site12 W 149.847805930032 S 17.5950858416171
site13 W 149.874295647818 S 17.5763485739245
site14 W 149.883782438236 S 17.5650093638768
site15 W 149.900771161799 S 17.5465400780548
site16 W 149.910261167288 S 17.5389997213247
site17 W 149.923545517018 S 17.5108598525766
site18 W 149.923940368089 S 17.4937812139175

Pictures from the field

photo_recrue

Note from the field

Total corals at the 27072023 (DMY)

1883

Site 1

  1. People: Arnaud Fabregues - Pierrick Harnay
  2. Transect Performed: T1 (20022023), T2 (20022023), T3 (21022023) T4 (21022023), T5 (21022023), T6 (21022023)
Coord GPS Name Longitude Latitude
Transect 1 Start POCS1T1S -17,485215 -149,87652
Transect 1 End POCS1T1E -17,484791 -149,87643
Transect 2 Start POCS1T2S -17,485172 -149,87637
Transect 2 End POCS1T2E -17,484828 -149,87619
Transect 3 Start POCS1T3S -17,485217 -149,87616
Transect 3 End POCS1T3E -17,484803 -149,87604
Transect 4 Start POCS1T4S -17,485291 -149,87602
Transect 4 End POCS1T4E -17,484888 -149,87586
Transect 5 Start POCS1T5S -17,485394 -149,87586
Transect 5 End POCS1T5EE -17,484923 -149,87574
Transect 6 Start POCS1T6S -17,485378 -149,87569
Transect 6 End POCS1T6E -17,484958 -149,87549
  1. Number of corals collected: T1 (22), T2 (7), T3 (26), T4 (4), T5 (5), T6 (6)
  2. Total number of samples: 70

XXXX

Site 2

  1. People: Arnaud Fabregues - Pierrick Harnay
  2. Transect Performed: T1 (21032023), T2 (23032023), T3 (27032023) T4 (28032023), T5 (31032023), T6 (XXX)
Coord GPS Name Longitude Latitude
Transect 1 Start      
Transect 1 End      
Transect 2 Start      
Transect 2 End      
Transect 3 Start      
Transect 3 End      
Transect 4 Start      
Transect 4 End      
Transect 5 Start      
Transect 5 End      
Transect 6 Start      
Transect 6 End      
  1. Number of corals collected: T1 (112), T2 (94), T3 (101), T4 (89), T5 (129), T6 (34)
  2. Total number of samples: 559

XXXX

Site 3

  1. People: Arnaud Fabregues - Pierrick Harnay
  2. Transect Performed: T1, T2, T3 (04022023) T4, T5, T6 (06102022)
Coord GPS Name Longitude Latitude
Transect 1 Start POCS3T1S -17,478309 -149,83423
Transect 1 End POCS3T1E -17,477992 -149,83407
Transect 2 Start POCS3T2S -17,47844 -149,83403
Transect 2 End POCS3T2E -17,477957 -149,83383
Transect 3 Start POCS3T3S -17,478423 -149,83392
Transect 3 End POCS3T3E -17,478033 -149,8337
Transect 4 Start POCS3T4S -17,47837 -149,83375
Transect 4 End POCS3T4E -17,477891 -149,83364
Transect 5 Start POCS3T5S -17,478408 -149,83354
Transect 5 End POCS3T5E -17,477972 -149,83339
Transect 6 Start POCS3T6S -17,478313 -149,83332
Transect 6 End POCS3T6E -17,478037 -149,83316
  1. Number of corals collected: T1 (41), T2 (17), T3 (26), T4 (33), T5 (9), T6 (8)
  2. Total number of samples: 134

XXXX

Site 4

  1. People: Dr Ariana Huffmyer - Danielle Becker - Pierrick Harnay
  2. Transect Performed: T1, T2, T3 (05102022) T4, T5, T6 (06102022) T7 (07102022)
Coord GPS Name Longitude Latitude
Transect 1 Start      
Transect 1 End      
Transect 2 Start      
Transect 2 End      
Transect 3 Start      
Transect 3 End      
Transect 4 Start      
Transect 4 End      
Transect 5 Start      
Transect 5 End      
Transect 6 Start      
Transect 6 End      
  1. Number of corals collected: T1 (36), T2 (26), T3 (29), T4 (20), T5 (43), T6 (43), T7 (7)
  2. Total number of samples: 204

XXXX

Site 5

  1. People: Arnaud Fabregues - Pierrick Harnay
  2. Transect Performed: T1 (07022023), T2 (12022023), T3 (15022023) T4 (16022023), T5 (09032023), T6 (18032023)
Coord GPS Name Longitude Latitude
Transect 1 Start POCS5T1S -17,472896 -149,78346
Transect 1 End POCS5T1E -17,472452 -149,78347
Transect 2 Start POCS5T2S -17,472958 -149,78334
Transect 2 End POCS5T2E -17,472558 -149,78318
Transect 3 Start POCS5T3S -17,473061 -149,78319
Transect 3 End POCS5T3E -17,472584 -149,78299
Transect 4 Start POCS5T4S -17,473068 -149,78299
Transect 4 End POCS5T4E -17,472633 -149,78293
Transect 5 Start POCS5T5S    
Transect 5 End POCS5T5E    
Transect 6 Start POCS5T6S    
Transect 6 End POCS5T6E    
  1. Number of corals collected: T1 (126), T2 (152), T3 (102), T4 (63), T5 (31), T6 (88)
  2. Total number of samples: 562

XXXX

Site 6

  1. People: Pierrick Harnay - Dr Hollie Putnam
  2. Transect Performed: T1 (02232024), T2 (02242024), T3 (02252024)
Coord GPS Name Longitude Latitude
Transect 1 Start      
Transect 1 End      
Transect 2 Start      
Transect 2 End      
Transect 3 Start      
Transect 3 End      
  1. Number of corals collected: T1 (88), T2 (74), T3 (77)
  2. Total number of samples: 239

XXXX

Site 7

  1. People: XXX - XXX
  2. Transect Performed: T1 (XXX), T2 (XXX), T3 (XXX) T4 (XXX), T5 (XXX), T6 (XXX)
Coord GPS Name Longitude Latitude
Transect 1 Start      
Transect 1 End      
Transect 2 Start      
Transect 2 End      
Transect 3 Start      
Transect 3 End      
Transect 4 Start      
Transect 4 End      
Transect 5 Start      
Transect 5 End      
Transect 6 Start      
Transect 6 End      
  1. Number of corals collected: T1 (X), T2 (X), T3 (X), T4 (X), T5 (X), T6 (X)
  2. Total number of samples: XX

XXXX

Site 8

  1. People: XXX - XXX
  2. Transect Performed: T1 (XXX), T2 (XXX), T3 (XXX) T4 (XXX), T5 (XXX), T6 (XXX)
Coord GPS Name Longitude Latitude
Transect 1 Start      
Transect 1 End      
Transect 2 Start      
Transect 2 End      
Transect 3 Start      
Transect 3 End      
Transect 4 Start      
Transect 4 End      
Transect 5 Start      
Transect 5 End      
Transect 6 Start      
Transect 6 End      
  1. Number of corals collected: T1 (X), T2 (X), T3 (X), T4 (X), T5 (X), T6 (X)
  2. Total number of samples: XX

XXXX

Site 9

  1. People: Pierrick Harnay - Dr Hollie Putnam
  2. Transect Performed: T1 (12222023), T2 (12242023), T3 (02292024)
Coord GPS Name Longitude Latitude
Transect 1 Start      
Transect 1 End      
Transect 2 Start      
Transect 2 End      
Transect 3 Start      
Transect 3 End      
  1. Number of corals collected: T1 (85), T2 (86), T3 (84)
  2. Total number of samples: 255

XXXX

Site 10

  1. People: XXX - XXX
  2. Transect Performed: T1 (XXX), T2 (XXX), T3 (XXX) T4 (XXX), T5 (XXX), T6 (XXX)
Coord GPS Name Longitude Latitude
Transect 1 Start      
Transect 1 End      
Transect 2 Start      
Transect 2 End      
Transect 3 Start      
Transect 3 End      
Transect 4 Start      
Transect 4 End      
Transect 5 Start      
Transect 5 End      
Transect 6 Start      
Transect 6 End      
  1. Number of corals collected: T1 (X), T2 (X), T3 (X), T4 (X), T5 (X), T6 (X)
  2. Total number of samples: XX

XXXX

Site 11

  1. People: Pierrick Harnay - Dr Hollie Putnam
  2. Transect Performed: T1 (180623), T2 (190623), T3 (XXX) T4 (12192023), T5 (12202023), T6 (12212023)
Coord GPS Name Longitude Latitude
Transect 1 Start      
Transect 1 End      
Transect 2 Start      
Transect 2 End      
Transect 3 Start      
Transect 3 End      
Transect 4 Start      
Transect 4 End      
Transect 5 Start      
Transect 5 End      
Transect 6 Start      
Transect 6 End      
  1. Number of corals collected: T1 (94), T2 (56), T3 (X), T4 (52), T5 (39), T6 (54)
  2. Total number of samples: 295

XXXX

Site 12

  1. People: XXX - XXX
  2. Transect Performed: T1 (XXX), T2 (XXX), T3 (XXX) T4 (XXX), T5 (XXX), T6 (XXX)
Coord GPS Name Longitude Latitude
Transect 1 Start      
Transect 1 End      
Transect 2 Start      
Transect 2 End      
Transect 3 Start      
Transect 3 End      
Transect 4 Start      
Transect 4 End      
Transect 5 Start      
Transect 5 End      
Transect 6 Start      
Transect 6 End      
  1. Number of corals collected: T1 (X), T2 (X), T3 (X), T4 (X), T5 (X), T6 (X)
  2. Total number of samples: XX

XXXX

Site 13

  1. People: XXX - XXX
  2. Transect Performed: T1 (XXX), T2 (XXX), T3 (XXX) T4 (XXX), T5 (XXX), T6 (XXX)
Coord GPS Name Longitude Latitude
Transect 1 Start      
Transect 1 End      
Transect 2 Start      
Transect 2 End      
Transect 3 Start      
Transect 3 End      
Transect 4 Start      
Transect 4 End      
Transect 5 Start      
Transect 5 End      
Transect 6 Start      
Transect 6 End      
  1. Number of corals collected: T1 (X), T2 (X), T3 (X), T4 (X), T5 (X), T6 (X)
  2. Total number of samples: XX

XXXX

Site 14

  1. People: XXX - XXX
  2. Transect Performed: T1 (XXX), T2 (XXX), T3 (XXX) T4 (XXX), T5 (XXX), T6 (XXX)
Coord GPS Name Longitude Latitude
Transect 1 Start      
Transect 1 End      
Transect 2 Start      
Transect 2 End      
Transect 3 Start      
Transect 3 End      
Transect 4 Start      
Transect 4 End      
Transect 5 Start      
Transect 5 End      
Transect 6 Start      
Transect 6 End      
  1. Number of corals collected: T1 (X), T2 (X), T3 (X), T4 (X), T5 (X), T6 (X)
  2. Total number of samples: XX

XXXX

Site 15

  1. People: Pierrick Harnay - Arnaud Fabregues - Jessica Tran
  2. Transect Performed: T1 (16032023), T2 (07262023), T3 (07282023) T4 (08052023)
Coord GPS Name Longitude Latitude
Transect 1 Start      
Transect 1 End      
Transect 2 Start      
Transect 2 End      
Transect 3 Start      
Transect 3 End      
Transect 4 Start      
Transect 4 End      
  1. Number of corals collected: T1 (83), T2 (121), T3 (134), T4 (62)
  2. Total number of samples: 400

XXXX

Site 16

  1. People: XXX - XXX
  2. Transect Performed: T1 (XXX), T2 (XXX), T3 (XXX) T4 (XXX), T5 (XXX), T6 (XXX)
Coord GPS Name Longitude Latitude
Transect 1 Start      
Transect 1 End      
Transect 2 Start      
Transect 2 End      
Transect 3 Start      
Transect 3 End      
Transect 4 Start      
Transect 4 End      
Transect 5 Start      
Transect 5 End      
Transect 6 Start      
Transect 6 End      
  1. Number of corals collected: T1 (X), T2 (X), T3 (X), T4 (X), T5 (X), T6 (X)
  2. Total number of samples: XX

XXXX

Site 17

  1. People: XXX - XXX
  2. Transect Performed: T1 (XXX), T2 (XXX), T3 (XXX) T4 (XXX), T5 (XXX), T6 (XXX)
Coord GPS Name Longitude Latitude
Transect 1 Start      
Transect 1 End      
Transect 2 Start      
Transect 2 End      
Transect 3 Start      
Transect 3 End      
Transect 4 Start      
Transect 4 End      
Transect 5 Start      
Transect 5 End      
Transect 6 Start      
Transect 6 End      
  1. Number of corals collected: T1 (X), T2 (X), T3 (X), T4 (X), T5 (X), T6 (X)
  2. Total number of samples: XX

XXXX

Site 18

  1. People: Pierrick Harnay - Dr Hollie Putnam
  2. Transect Performed: T1 (12112023), T2 (12272023) only 48.2m because Eel bite, T3 (02282024)
Coord GPS Name Longitude Latitude
Transect 1 Start      
Transect 1 End      
Transect 2 Start      
Transect 2 End      
Transect 3 Start      
Transect 3 End      
  1. Number of corals collected: T1 (50), T2 (33), T3 (51)
  2. Total number of samples: 134

XXXX

Written on September 19, 2022