Relative abundance Pocillopora spp. around the island of Mo’orea

Context:

Our study is based on population genetics of Pocillopora sp. on the Polynesian islands based on their often misleading and incorrect morphology. We are therefore trying to understand population dynamics by Haplotype so that we can correlate our transect data with our term tolerance data from the laboratory (see NoteBook TPC Haplotype).

PCR after extracion and nano drop control:

Protocol:

Gel process control of DNA quality

Protocol:

During the process we used differentes size of gel. For this raison we have list bellow all the different way to make the gel at 1.5%.

Small size of gel:

• 1.12g of Agarose
• 75ml of 1x TAE Buffer
• 1µl of gelgreen.

Medium size of gel:

• 1.50g of Agarose
• 100ml of 1x TAE Buffer
• 1µl of gelgreen.

Large size of gel:

• 2.25 of Agarose
• 150ml of 1x TAE Buffer
• 1µl of gelgreen.

On each gel add 4µl of lader on one side of the gel. On each gel add 4µl of samples in each well. Run the gel at 100V during 35 minutes.