Equipment and Reagents

• Sample Preservation and Lysis Zymo Research DNA/RNA Shield R1100-250
• DNA Extraction Zymo Research Quick-DNA™ Miniprep Plus Kit D4069
• Foward primer FatP6.1 200µM Stock IDT)
• Reverse primer RORF 200µM Stock IDT
• Master Mix EmeraldAmp GT PCR Master Mix
• EmeraldAmp GT PCR Master Mix is a loading-dye-added version of EmeraldAmp MAX PCR Master Mix that is optimized for great performance and convenience in both standard and high-throughput PCR applications
• Loading Dye NEB 6X Purple Loading Dye NEB Cat # B7024S
• Gel Stain Biotium GelGreen Nucleic Acid Gel Stain, 10,000X in Water Fisher Cat NC9728313
• DNA Ladder 1kb Gel ladder
• Gel ladder GeneRuler 100 bp DNA Ladder (Thermo FIsher Catalog SM0241)
• Restriction Enzyme XhoI R0146Sk
• Restriction Enzyme Buffer rCutSmart B6004S